Coding
Part:BBa_K3425041:Design
Designed by: Tereza Hubackova Group: iGEM20_UofUppsala (2020-10-20)
HSP 16.3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 177
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is codon optimised for E. coli.
This part is designed for the
Type IIS iGEM standard assembly
and therefore the coding sequence lacks the start codon. In Type IIS standard assembly, the ATG start codon is included in the coding-sequence prefix, as shown in the template CDS part
(BBa_K3425019).
You can also find useful design templates and more information about how to use this standard in the Type IIS guidebook
of Uppsala 2020 team.
Source
Synthesized. The sequence originates from Mycobacterium tuberculosis.